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By F. F. Nord

Advances in Enzymology and similar parts of Molecular Biology is a seminal sequence within the box of biochemistry, delivering researchers entry to authoritative stories of the most recent discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, offering an unmatched view of the ancient improvement of enzymology. The sequence bargains researchers the newest knowing of enzymes, their mechanisms, reactions and evolution, roles in advanced organic method, and their program in either the laboratory and undefined. every one quantity within the sequence gains contributions by means of top pioneers and investigators within the box from all over the world. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.

With its wide selection of themes and lengthy ancient pedigree, Advances in Enzymology and comparable components of Molecular Biology can be utilized not just by way of scholars and researchers in molecular biology, biochemistry, and enzymology, but additionally by means of any scientist drawn to the invention of an enzyme, its houses, and its functions.

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J. Extract with baryta a t room temperature Centrifugate ............................................... 0 Filtrate I PreciDitate with barium acetate Preci- itate Wash exhaustively with hot water Decompose in normal sulfuric acid.. . . . . . . . . . Extract with liquid phenol Mother liquor Saturate with ammonium sulfate Extract with liquid phenol Phenol extract J. Extract with water and ether mixture Water phase.. Acidify to pH 1 with nitric acid Extract with isobutanol Isobutanol extract J.

It is a plain fact that the first requisite for a drug to show antibacterial action is that it enter into combination with one or more vital groups of the bacterial cell. Whatever may be the exact nature of this combination, the fact remains that the above physicochemical explanations do not give us a hint regarding the chemical nature of the enzymes which play a vital ENZYME PROBLEMS IN RELATION TO CHEMOTHERAPY 37 part in the biology of bacteria and are specifically inhibited by these drugs. Why the other enzyme proteins are not similarly affected, despite the fact that as proteins they must have ionizable hydrogens, cannot be answered by these theories.

VIII. Conclusions.. . . . . . . . . . . . . . . . . . . . . . . . . . . Bibliography. . . . ..... . 90 90 91 96 102 113 118 119 121 I. Introduction An understanding of the problems indicated by the title of the present study requires their treatment in relation to one another. By emphasizing the biochemical aspects involved, it is hoped that some light may be thrown on the chemical nature of various hitherto undefined biological processes.

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