By Davide Ricci, Pier Carlo Braga
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Additional info for Atomic Force Microscopy: Biomedical Methods and Applications (Methods in Molecular Biology Vol 242)
Barrett, R. C. and Quate, C. F. (1991) Optical scan-correction system applied to atomic force microscopy. Rev. Sci. Instrum. 62, 1393–1399. 38 Ricci and Braga Micromechanical Biosensors 39 4 Advanced Biosensing Using Micromechanical Cantilever Arrays Martin Hegner and Youri Arntz 1. Introduction During the last few years, a series of new detection methods in the field of biosensors have been developed. Biosensors are analytical devices that combine a biologically sensitive element with a physical or chemical transducer to selectively and quantitatively detect the presence of specific compounds in a given external environment.
Microsc. Res. Tech. 44, 312–326. 10. Dinte, B. , Watson, G. , Dobson, J. , and Myhra, S. (1996) Artefacts in noncontact mode force microscopy: The role of adsorbed moisture. Ultramicroscopy 63, 115–124. 11. , and Shao, Z. (1996) The effect of deformation on the lateral resolution of the atomic force microscopy. J. Microsc. 182, 106–113. 12. van Noort, S. , van der Werf, K. , de Grooth, B. , van Hulst, N. , and Greve, J. (1997) Height anomalies in tapping mode atomic force microscopy in air caused by adhesion.
It is preferable to grow the cell culture directly in a culture dish that will then constitute the fluid cell for the Explorer Stage. Several of the AFM manufacturers now offer comparably equipped standalone instruments or equivalent optional attachments. The preferred scanner for the latter procedure should have a maximum field of view of some 130 × 130 µm2 and a z range of 10 µm. All combinations of instrumentation will accommodate the full range of relevant operational modes: contact, in situ intermittent-contact in fluid, and F-d analysis.