By John H. Rothman, Andrew Singson
An up to date version of the classic Methods in mobilephone Biology quantity forty eight, this book emphasizes various tools and applied sciences had to examine C. elegans, either as an built-in organism and as a version approach for study inquiries in cellphone, developmental, and molecular biology, in addition to in genetics and pharmacology. by means of directing its audience to tried-and-true and state of the art recipes for research, this accomplished assortment is meant to steer investigators of C. elegans for future years. *Diverse, updated thoughts covered will be invaluable to the broadening neighborhood of C. elegans researchers for years yet to come* Chapters written through leaders within the box* attempted and precise equipment carry busy researchers a one-stop compendium of crucial protocols
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Extra info for Caenorhabditis elegans: Molecular Genetics and Development
1990). fog-1, a regulatory gene required for specification of spermatogenesis in the germ line of Caenorhabditis elegans. Genetics 125, 29–39. Brenner, S. (1974). The genetics of Caenorhabditis elegans. Genetics 77, 71–94. , and Strome, S. (1996). A sperm-supplied factor required for embryogenesis in C. elegans. Development 122, 391–404. Bullerjahn, A. , and Riddle, D. L. (1988). Fine-structure genetics of ama-1, an essential gene encoding the amanitin-binding subunit of RNA polymerase II in Caenorhabditis elegans.
In some cases, half-translocations can be maintained as extrachromosomal duplications and may in these cases be given a Dp designation. For example, a half-translocation from the reciprocal translocation hT1(I;V) that was isolated in the Rose laboratory at the University of British Columbia (h), recombines and segregates from chromosome I. It can be maintained as a duplication of regions of chromosomes I and V in addition to the normal diploid complement and has been designated hDp133, rather than the precise but more cumbersome hT1(IRVL).
Jones et al. 5. Single-Gene Deletions Single-gene deletions, often referred to as gene knockouts, are valuable tools for determining gene function. Knockouts are generated using mutagens that create small deletions, most commonly trimethyl psoralen (TMP) followed by UV-radiation (UV-TMP). In C. elegans, there is an international effort to generate gene knockouts for the research community (Barstead and Moerman, 2006; Mitani, 2009; Moerman and Barstead, 2008). org). Requests to have a knockout allele generated for a specific gene should be sent to either the National Bioresource Project, Tokyo Women’s Medical University School of Medicine, Japan (http://www.