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The present data therefore indicate that the neonatal period represents a stage where B-lymphocytes probably have not as yet differentiated to any large extent into mature plasma cells (Froland and Natvig, 1972b). D. RESTRICTION OF MEMBRANE-BOUND IMMUNOGLOBULINS ON SINGLELYMPHOCYTES The accumulated evidence indicates that each normal lymphocyte stains only for one immunoglobulin class. , 1971; Froland and Natvig, 1971, 1972a). However, also in double staining experiments, the main iminunoglobulin classes and also thc four IgG subclasses were represented HUMAN IMMUNOGLOBULINS 35 on different normal lymphocytes ( Froland and Natvig, 1 9 7 2 ~ ) In .

4. Amino Acid Sequences a d Their Relutionship to Subclasses and Genetic Markers The whole amino acid sequence of one IgGl Gm( f ) molecule is now known, and several stretches of other IgG subclasses are characterized. , 1972b). There are also striking differences in the interchain disulfide bridges (Milstein and Pink, 1970). However, as yet no complete study has been made of all the IgG subclasses and their genetic variants. From amino acid interchanges in certain positions, from peptide mapping analysis, and the relationship of certain antigens to structural subfragments, good evidence is available for some amino acids being directly related to specific antigenic determinants.

RESTRICTION OF MEMBRANE-BOUND IMMUNOGLOBULINS ON SINGLELYMPHOCYTES The accumulated evidence indicates that each normal lymphocyte stains only for one immunoglobulin class. , 1971; Froland and Natvig, 1971, 1972a). However, also in double staining experiments, the main iminunoglobulin classes and also thc four IgG subclasses were represented HUMAN IMMUNOGLOBULINS 35 on different normal lymphocytes ( Froland and Natvig, 1 9 7 2 ~ ) In . the latter experiments it was also shown that one lymphocyte only carried the allele of one subclass gene and it appeared that the lymphocytes showed allelic exclusion as to their surface-bound immunoglobulins.

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