Download Diagnostic Bacteriology Protocols 2nd Edition (Methods in by Louise O'Connor PDF

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702, 61–68. 37. Mock, K. (1993) Routine sensitive peptide mapping using LC/MS of therapeutic proteins produced by recombinant DNA technology. Pept. Res. 6, 100–104. 38. , Daae, F. , Sandaa, R. , and Ovreas, L. (1998) Novel techniques for analysing microbial diversity in natural and perturbed environments. J. Biotechnol. 64, 53–62. 39. Lindahl, T. (1997) Facts and artifacts of ancient DNA. Cell 76, 49–99. 40. , and Rudi, K. (2000) Application of the 5'nuclease PCR assay in the evaluation and development of methods for quantitative detection of Campylobacter jejuni.

While freely diffusible primers are deployed for amplification, the nested PCR is initiated by oligonucleotide primers bound to a solid phase. Thus, on-chip PCR allows the single-step amplification and characterization of a DNA sample as a result of separation in liquid- and solid-phase reactions. In contrast to conventional PCR, the reaction is performed directly on the flat surface of a glass slide that holds an array of covalently attached nested primers. The bacterial target DNA is amplified and probed using primers identifying either species-specific sequence regions of ribosomal DNA or unique bacterial target genes, such as virulence or resistance factors.

Clin. Microbiol. 186, 4228–4237. 47. Spratt, B. G. , and Johnson, A. ), Humana, Totowa, NJ, pp. 323–352. 48. Fredricks, D. N. and Relman, D. A. (1996) Sequence-based identification of microbial pathogens: a reconsideration of Koch’s Postulates. Clin. Microbiol. Rev. 9, 18–33. 49. , Scheld, H. , and Becker, K. (2005) Impact of a molecular approach to improve the microbiological diagnosis of infective heart valve endocarditis. Circulation. 111, 1415–1421. 50. Valerius, N. , and Hoiby, N. (1991) Prevention of chronic Pseudomonas aeruginosa colonization in cystic fibrosis by early treatment.

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