Download Genomics Protocols by Kaisa Silander, Janna Saarela (auth.), Mike Starkey, Ramnath PDF

By Kaisa Silander, Janna Saarela (auth.), Mike Starkey, Ramnath Elaswarapu (eds.)

Since the 1st version, released in 2001, genomics examine has taken nice strides. In Genomics Protocols, 2d Edition, a group of professional researchers proportion the most up-tp-date details in a box that has lately switched emphasis from gene identity to sensible genomics and the characterization of genes and gene items. This quantity techniques its complicated topic with a wide viewpoint to provide its reader with an important assessment of genomics and its spinoff fields, with a spotlight on pivotal matters equivalent to facts research. Following the hugely profitable equipment in Molecular Biology™ sequence layout, the chapters offer easy-to-follow laboratory protocols, lists of the required fabrics, and the superb Notes part, which bargains tips about troubleshooting and fending off recognized pitfalls.

Expansive and present, Genomics Protocols, 2d Edition is the correct replace for researchers operating during this necessary, growing to be field.

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Nucleic Acids Res 13:3111–3129 14. Sheffield VC, Cox DR, Lerman LS, Myers RM (1989) Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc Natl Acad Sci USA 86:232–236 15. Khrapko K, Hanekamp, JS, Thilly WG, Belenkii A, Foret F, Karger BL (1994) Constant denaturant capillary electrophoresis (CDCE): A high resolution approach to mutational analysis. Nucleic Acids Res 22:364–369 16.

9. Gel electrophoresis equipment. 10. 0. Make up the vol to 1 L with water (Sigma Aldrich, 93290-1 L). 11. Electrophoresis-grade agarose (BioRad, 161-3101 [125 g]). 12. Ethidium bromide (Sigma Aldrich, E8751-5G). 13. Agilent bioanalyser and DNA chips (Agilent, 5067-1506). 14. Gel Extraction Kit (Qiagen, 28704 ). 15. QIAquick PCR Purification Kit (Qiagen, 28104). 16. Incubating shaker. 17. Thermal cycler for PCR amplification. 18. 15-mL Falcon tubes (Fisher, CFT-420-079D). 2 Setting up Sequencing Reactions 19.

Alternatively, load 1 µL of each PCR product into the wells of a DNA 7500 chip and run on the Agilent Bioanalyser following the protocol provided with the instrument. 39. If a single band is present, purify the PCR product using Qiagen PCR Purification columns, following the protocol provided. If more than one band is seen, load the remaining PCR sample onto a 1% TBE agarose gel, containing ethidium bromide. Purify the correct band by cutting out of the gel with a scalpel blade and use a Qiagen Gel Extraction Kit to isolate DNA from agarose slices (see Note 2).

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