By N. Harris (editor), D. G. Wilkinson (editor)
Advances in our realizing of organic mechanisms have often been linked to the improvement of latest recommendations. In situ hybridization is a vintage case of simply such an increase. The method successfully combines histochemistry with molecular biology and permits the quick research of the distribution of RNA, or DNA, within the tissues. the data received from this has prompted anything of a revolution in our figuring out of developmental biology, because a basic point of improvement is the spatial and temporal expression of genes. additionally the strategy has discovered software within the box of medication, the place it really is giving new insights into the functioning of fit tissues and the prognosis and examine of illnesses. This booklet brings jointly contributions from leaders within the software of this method and should support the reader assessment and be guided in the course of the a number of techniques and diversifications of the approach.
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Extra info for In Situ Hybridisation: Application to Developmental Biology and Medicine (Society for Experimental Biology Seminar Series (No. 40))
C. , Raap, A. , Beck, J. L. , & Vooij, G. P. (1988). In situ hybridization as a tool to study numerical chromosome aberrations in solid bladder tumors. Histochemistry, 89, 307-16. Jiwa, N. , Raap, A. , van de Rijke, F. , Zwaan, F. ,The,T. H. & van der Ploeg, M. (1989). Detection of human cytomegalovirus antigens in formaldehyde fixed material from bone marrow transplant patients. Journal of Clinical Pathology, in press. , Birnstiel, M. & Jones K. (1969). RNA-DNA hybrids at the cytological level.
Pardue, M. L. (1969). Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proceedings of the National Academy of Sciences, USA, 63, 378-81. Gall, G. & Pardue, M. L. (1971). Nucleic acid hybridization in cytological preparations. Methods in Enzymology, 38, 470-80. Garson, J. , van den Berghe, J. A. & Kemshead, J. T. (1987). Novel nonisotopic in situ hybridization technique detects small (1 kb) unique sequences in routinely G-banded human chromosomes: fine mapping of ~N-myc and beta-M/F genes.
Singer, R. H. (1985). Quantitative analysis of in situ hybridisation for the detection of actin gene expression. Nucleic Acids Research, 13 (5), 1777-99. Lawrence, J. B. & Singer, R. H. (1986). Intracellular localization of messenger RNAs for cytoskeletal proteins. Cell, 45, 407-15. Lewis, M. , Sherman, T. G. & Watson, S. J. (1985). In situ hybridisation with synthetic oligonucleotides: strategies and methods. Peptides, 6, Suppl. 2, 75-87. , Grosveld, F. & Rechardt, L. (1986). Specific detection of neuronal cell bodies: in situ hybridization with a biotinlabeled neurofilament cDNA probe.