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By Albert L. Latner

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1 7 . G e n e t i c v a r i a n t s of isocitrate d e h y d r o g e n a s e ( r e p r o d u c e d w i t h permission from H e n d e r s o n , 1 9 6 5 ) . 46 ISOENZYMES IN BIOLOGY AND MEDICINE s t r u c t u r e for t h e e n z y m e has b e e n p r o p o s e d ( H e n d e r s o n , 1965). T w o p o l y p e p t i d e sub-units sa a n d sb forming d i m e r s giving t h e active isoenzymes sa-sa, s b - s b , a n d s a - s b h a v e b e e n suggested. I n t h e h é t é r o zygote, all t h r e e isoenzymes could b e d e t e c t e d .

G L U T A M A T E DEHYDROGENASE U s i n g agar-gel electrophoresis a n d a visual staining m e t h o d , it has b e e n possible to s e p a r a t e t h e g l u t a m a t e d e h y d r o g e n a s e of h u m a n tissues i n t o five fractions; t h e r e a p p e a r to be differences in t h e isoenzyme p a t t e r n s from different tissues ( V a n d e r H e l m , 1962b). 6. GLUCOSE-6-PHOSPHATE DEHYDROGENASE T h i s e n z y m e was s e p a r a t e d i n t o m u l t i p l e forms b y starch-gel electrophoresis ( T s a o , 1960).

S t a r c h gel electrophoresis has b e e n used to fractionate t h e m a l a t e d e h y d r o g e n a s e activity in v a r i o u s c r u d e a n i m a l tissue extracts ( M a r k e r t a n d Moller, 1959). A t least t w o b a n d s could be d e t e c t e d . Similar results h a v e b e e n r e p o r t e d b y T s a o (1960) a n d L a t n e r a n d Skillen (1962). T w o m a l a t e d e h y d r o g e n a s e s h a v e b e e n d e m o n s t r a t e d in h u m a n s p e r m a t o z o a b y a c r y l a m i d e gel electrophoresis ( G o l d b e r g , 1963).

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