By Ormond A Macdougald
Methods of Adipose Tissue Biology is a must have for somebody attracted to weight problems or the body structure of white or brown adipose tissues. It includes state of the art equipment from researchers who're international leaders during this box. specific lab protocols comprise ways to visualize adipocytes and adipose tissues in people and experimental types, changing stem cells into white and brown adipocytes in vitro, comparing elements of adipocyte metabolism, inducibly knocking out genes in adipose tissues, and comparing transcriptional keep an eye on of adipogenesis on an international scale.
- The research of adipose tissue is going hand in hand with our worldwide attempt to appreciate and opposite the epidemic of weight problems and linked clinical complications
- Contributors contain major researchers who've made great contributions to our skill to enquire white and brown adipose tissues
- The large choice of experimental ways distinct inside this quantity: together with the evaluate of adipose tissue biology on the molecular, biochemical, mobile, tissue, and organismal levels
Read or Download Methods of Adipose Tissue Biology, Part A PDF
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Additional resources for Methods of Adipose Tissue Biology, Part A
Sample fluorescent-minus-one (FMO) controls for each fluorochrome.
Several stromal cell populations comprise the nonadipocyte cell populations, including endothelial, blood, and mesenchymal cell populations. As mature adipocytes are terminally differentiated postmitotic cells, the differentiation of adipocyte precursors is necessary for the establishment of adipocyte number during development and expansion in obesity (Herberg, Do¨ppen, Major, & Gries, 1974; Hirsch & Batchelor, 1976). , 2008). It has long been known that adipocyte precursors reside within WAT depots as culturing of the heterogeneous mixture of WAT resident stromal cells, termed the stromal vascular fraction (SVF), results in the generation of lipid-filled adipocytes (Ng, Poznanski, Borowiecki, & Reimer, 1971).
These cells are enriched for adipogenic expression markers, such as Pparg2, and are capable of forming adipocytes when transplanted outside of the WAT microenvironment, Isolation and Study of Adipocyte Precursors 33 suggesting further commitment to the adipocyte lineage (Berry & Rodeheffer, 2013), In this chapter, we provide an overview of methods utilized to identify, analyze, and isolate these adipocyte precursor cell populations from murine WAT. We also detail protocols for isolation by fluorescent-activated cell sorting (FACS) or analysis by flow cytometry.