By Thomas A. Owen, Lydia C. Pan (auth.), Jennifer J. Westendorf (eds.)
In contemporary years, the research of bone cells and tissues on the mobile and molecular degrees in numerous versions has revolutionized the sector. In Osteoporosis: equipment and Protocols, best scientists from all over the world proportion their step by step laboratory protocols for learning bone biology. the subjects lined during this quantity comprise in vitro types, in vivo types applied for drug trying out, tissue engineering and osteoporosis stories in both gender, cutting-edge molecular ideas to evaluate unmarried genes or for worldwide genomic research, strong imaging concepts, and plenty of extra. As a quantity within the hugely profitable Methods in Molecular Biology™ sequence, each one bankruptcy presents a short advent, an inventory of useful fabrics, and a Notes part detailing pointers on troubleshooting and averting identified pitfalls.
Comprehensive and state-of-the-art, Osteoporosis: equipment and Protocols is the appropriate consultant for either new and skilled experimentalists trying to examine the devastating silent sickness referred to as osteoporosis.
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Additional resources for Osteoporosis: Methods and Protocols
V. (1966). Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol 16, 381–390. Peck, W. , Birge, S. J. , Fedak, S. A. (1964). Bone cells: biochemical and biological studies after enzymatic isolation. Science 146, 1476–1477. , Cohn, D. V. (1974). Separation of parathyroid hormone and calcitonin-sensitive cells from non-responsive bone cells. Nature 252, 713–715. Aubin, J. E. (1999). Osteoprogenitor cell frequency in rat bone marrow stromal populations: role for heterotypic cell-cell interactions in osteoblast differentiation.
2 Osteoclast Culture and Resorption Assays 35 14. , et al. (1986) Scanning electron microscopy in bone pathology: review of methods, potential and applications. Scan Electron Microsc Pt 4, 1537–1554. 15. Onyia, J. , et al. (2004) Novel and selective small molecule stimulators of osteoprotegerin expression inhibit bone resorption. J Pharmacol Exp Ther 309, 369–379. 16. , et al. (1988) Human parathyroid hormone (PTH)-related protein and human PTH: comparative biological activities on human bone cells and bone resorption.
6. Remove the marrow precursors by snipping the ends of each bone to expose the marrow cavity using sterile scissors and forceps. Using a 27-gauge needle and syringe, flush the marrow cavity with sterile PBS to remove precursors. 7. Pellet the cells by centrifugation at 1,000 g for 10 minutes. Aspirate the supernatant and suspend the cells in 5 mL RBC lysis buffer for 5 minutes (see Note 2). Pellet cells by centrifugation at 1,000 g for 10 minutes. 8. 9 × 107 cells in 8 mL in αMEM supplemented with 25 ng/mL M-CSF (80-µL) per dish.