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Extra info for Phase II Conjugation Enzymes and Transport Systems
Novel functional polymorphisms in the UGT1A7 and UGT1A9 glucuronidating enzymes in Caucasians and African‐American subjects and their impact on the metabolism of 7 ethyl‐ 10‐hydroxycamptothecin and flavopiridol anticancer drugs. J. Pharmacol. Exp. Ther. 307, 117–128. Wells, P. , Mackenzie, P. , Chowdhury, J. , Gregory, P. , Hansen, A. , Kessler, F. , Kim, P. , Chowdhury, N. , and Ritter, J. K. (2004). Glucuronidation and the UDP‐glucuronosyltransferases in health and disease. Drug Metab. Disp. 32, 281–290.
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5 g of pGL3‐basic or the pGL3‐promoter constructs using 2–4 l of Lipofectamine2000, according to the supplied protocol. Twenty‐five nanograms of pRL‐Null plasmid (Promega), which expresses low constitutive levels of the Renilla luciferase gene, is also included in every transfection to serve as the internal reference. Other members of the pRL vector family (Promega) are also suitable for use as internal control reporters; the suitability of each will depend on the cell lines and experimental conditions chosen.