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This new capability was reported to be completely abolished by heating. The latter results 12 B. SINGER AND H. FRAENKEL-CONRAT would seem to givestrong support to the authors’ preferred interpretation of the data, namely that the hydration of the 5,6 double bond of cytosine represents the mutagenic event, possibly by causing the tautomeric shift at N-3,C-4. However, if hydration of the double bond caused the tautonieric shift of amino to imino in cytosine, one could hardly expect the same reaction to cause the opposite shift, keto to enol, in uracil to account for the serine coding activity detected by Grossman in irradiated poly U.