By Paul Popescu, Helene Hayes, Bernard Dutrillaux, R. Popescu
The theoretical history of cytogenetics in addition to the sensible tips on how to examine chromosomes from Drosophila to mammals and people are provided during this publication. concept and functional protocols at the following issues are incorporated: telephone tradition tools, chromosome guidance and banding suggestions, radioactive and fluorescent in situ hybridisation (FISH) tools and their derivates (chromosome painting), meiotic chromosome equipment utilizing gentle and digital microscopy, the polytenic (Drosophila) and lampbrush (urodeles) chromosomes in addition to move and slit test cytometry. The editors, all at INRA, performed a renowned half within the improvement of cytogenetics. Paul Popescu, a consultant for family animals labored with gene mapping, Helene Hayes, including Popescu confirmed the homology of bovine and human chromosome problems, and Bernard Dutrilaux fascinated with human cytogenetics.
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Extra info for Techniques in Animal Cytogenetics
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The cells are then fixed in fresh fixative (3 vol. et hanoi - 1 vol. acetic acid) for at least one hour Chromosome spreads are then prepared as for mammalian cells. S g of body weight of a colchicine solution (10 /lg/ml appendix V2), either intraperitoneally or into the wing vein of the bird). From 1 to 11/2 hours later, feather pulp is collected and cells extracted from it are incubated directly in the hypotonic solution without culture. Bird sex can be determined by chromosome analysis in most species.
When the cells become confluent, it is necessary to collect them via a trypsin treatment and to subculture them as described above. This operation is referred to as one passage. Remarks Different foetal tissue cells can be cultured. However for pig, domestic cattle, sheep and goat, it has been frequently observed that lung cells give the best results. These cells can be maintained in culture for approximately 15 to 20 passages, but after 7 to 10 passages, they become less active and their mitotic index decreases.