Download Techniques in Animal Cytogenetics by Paul Popescu, Helene Hayes, Bernard Dutrillaux, R. Popescu PDF

By Paul Popescu, Helene Hayes, Bernard Dutrillaux, R. Popescu

The theoretical history of cytogenetics in addition to the sensible tips on how to examine chromosomes from Drosophila to mammals and people are provided during this publication. concept and functional protocols at the following issues are incorporated: telephone tradition tools, chromosome guidance and banding suggestions, radioactive and fluorescent in situ hybridisation (FISH) tools and their derivates (chromosome painting), meiotic chromosome equipment utilizing gentle and digital microscopy, the polytenic (Drosophila) and lampbrush (urodeles) chromosomes in addition to move and slit test cytometry. The editors, all at INRA, performed a renowned half within the improvement of cytogenetics. Paul Popescu, a consultant for family animals labored with gene mapping, Helene Hayes, including Popescu confirmed the homology of bovine and human chromosome problems, and Bernard Dutrilaux fascinated with human cytogenetics.

Show description

Read or Download Techniques in Animal Cytogenetics PDF

Similar basic science books

The Nuts and bolts of Paced ECG Interpretation (Nuts and Bolts Series)

Not anything is extra puzzling to the clinician new to equipment remedy than having to accommodate cardiac electrocardiograms from a tool sufferer. Pacemakers and different implantable cardiac rhythm administration units depart their “imprint” on ECGs and will considerably switch what clinicians see - or count on to determine.

Computational Fluid and Particle Dynamics in the Human Respiratory System

Conventional examine methodologies within the human breathing approach have continuously been tough as a result of their invasive nature. contemporary advances in clinical imaging and computational fluid dynamics (CFD) have sped up this examine. This publication compiles and info fresh advances within the modelling of the breathing procedure for researchers, engineers, scientists, and well-being practitioners.

Excitation-Contraction Coupling and Cardiac Contractile Force

The most objective of this monograph is to supply an outline of calcium rules in cardiac muscle cells, relatively with admire to excitation-contraction coupling and the keep an eye on of cardiac contractile strength. it really is my desire that this e-book should be helpful to scholars of the cardiovascular approach and muscle in any respect assorted degrees and in several disciplines (such as body structure, biochemistry, pharmacology and pathophysiology).

NMR in Physiology and Biomedicine

This booklet offers a accomplished evaluation of contemporary nuclear magnetic resonance ways to biomedical difficulties in vivo utilizing cutting-edge suggestions. It devotes equivalent cognizance to the equipment and functions of NMR and addresses the possibility of all of the recommendations mentioned. the amount comprises late-breaking components corresponding to sensible imaging, movement imaging, bioreactor spectroscopy, and chemical shift imaging.

Extra info for Techniques in Animal Cytogenetics

Example text

16 21 •• 26 7 8 . 12 ' 17 . , 22 lei I. ~ .. 13 il 18 .... 2.

The cells are then fixed in fresh fixative (3 vol. et hanoi - 1 vol. acetic acid) for at least one hour Chromosome spreads are then prepared as for mammalian cells. S g of body weight of a colchicine solution (10 /lg/ml appendix V2), either intraperitoneally or into the wing vein of the bird). From 1 to 11/2 hours later, feather pulp is collected and cells extracted from it are incubated directly in the hypotonic solution without culture. Bird sex can be determined by chromosome analysis in most species.

When the cells become confluent, it is necessary to collect them via a trypsin treatment and to subculture them as described above. This operation is referred to as one passage. Remarks Different foetal tissue cells can be cultured. However for pig, domestic cattle, sheep and goat, it has been frequently observed that lung cells give the best results. These cells can be maintained in culture for approximately 15 to 20 passages, but after 7 to 10 passages, they become less active and their mitotic index decreases.

Download PDF sample

Rated 4.17 of 5 – based on 44 votes