Download Tetrahymena thermophila by David J Asai; James D Forney; American Society for Cell PDF

By David J Asai; James D Forney; American Society for Cell Biology

Tetrahymena thermophila is rising as a strong experimental method for useful genetics. The ciliated protozoan bargains a number of merits, no longer the least of that's the power to do away with any particular gene of curiosity after which to judge the impression of that mutation at the dwelling mobilephone. prior investigations with T. thermophila have yielded a number of key discoveries, together with dynein, catalytic RNA, and telomerase. This quantity is a complete source for utilizing Tetrahymena within the laboratory and is aimed toward folks already skilled, in addition to beginners to the organism. It covers either the organic history and crucial protocols for investigators quickly turning to Tetrahymena because the experimental approach of selection.

Key Features
* includes either theoretical and functional concerns in 30 chapters contributed by means of the realm experts on Tetrahymena
* Indispensible for either the beginner and the skilled researcher
* Overviews the heritage, mobile biology, and genetics of the organism
* Describes crucial protocols at the development of cells, genetic options, and the way to examine the cells with the microscope
* Illustrates how the equipment will be utilized to resolve quite a few phone organic problems
* reports lately built recommendations for changing gene expression

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Additional resources for Tetrahymena thermophila

Example text

Cell Biology of Tetrahymrna themophila 39 Fig. 5 A cutaway diagram of a portion of the cell surface of Tetruhyrnenu, showing segments of two ciliary rows and their associated fibrillar elements. The anterior end of the cell is oriented upward in the diagram, receding from the viewer. The surface of the plasma membrane, with protruding cilia (C) and docking sites of the mucocysts is shown in the more anterior portion. Coated pits are omitted. The membrane is sectioned to show the cortical alveoli (Alv) and one mucocyst (Mc) (= dense core granule).

33,59-70. Schensted, I. (1958). Appendix: Model of subnuclear segregation in the macronucleus of ciliates. Am. Nut. 92,161-170. , and Zeuthen, E. Induction of synchronous cell division in mass cultures of Tetrahymena pyriformis. Exp. Cell Res. 6,221-227. , and Willis, J. H. Molecular polymorphisms of ciliary proteins from different species of the ciliate Tetrahymena. Biochem Genet. 19,385-3%. M. Freezing and storage in liquid nitrogen of axenically and monoxenically cultivated Tetrahymena pyriformis.

Am. Microsc. SOC. 9 4 155-178. van Wagtendonk, W. J. (1974). Nutrition in Paramecium. In “Paramecium: A Current Survey” (W. J. ), pp. , Amsterdam. Weindruch, R. , and Doerder, F. P. Mech. Ageing Dev. 4263-279. Williams, N. E. (1986). An apparent disjunction between the evolution of form and substance in Tetrahymena. Evolution (Lawrence, KS) 38,25-33. Williams, N. , Buhse, H. , and Smith, M. Protein similaritiesin the genus Tetrahymena and a description of Tetrahymena leucophrys, n. sp. J. Protozool.

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