Download The Leukemia-Lymphoma Cell Line FactsBook by Hans G. Drexler PDF

By Hans G. Drexler

The Leukemia-Lymphoma cellphone Line Factsbook represents a vital reference guide for the entire well-characterized leukemia-lymphoma mobile traces at the moment to be had. It offers crucial evidence, utilizing the succinct and trouble-free layout that has made the FactsBooks so well-liked by scientists and medical researchers. Introductory chapters supply history and point of view for culturing malignant hematopoietic (blood forming) telephone traces. those chapters are by way of over four hundred accomplished person entries. each one mobile line access highlights crucial scientific, immunological, genetic, and practical good points and incorporates a complete directory of references.

  • The complete spectrum of malignant telephone strains from all hematopoietic cellphone lineages
  • Sister phone strains and suitable subclones
  • Clinical facts: sufferer, prognosis, therapy prestige, and specimen source
  • Authentication of derivation and availability
  • Immunophenotype
  • Cytogenetic karyotype
  • Translocations and fusion genes
  • Receptor gene rearrangements and genetic alterations
  • Cell cultures features: institution, medium, doubling time, growth
  • Cytochemical profile
  • Cytokine creation and reaction to cytokines
  • Proto-oncogene and transcription issue expression/alteration
  • Functional gains: differentiation induction, heterotransplantability
  • Special special features
  • Key references

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Extra info for The Leukemia-Lymphoma Cell Line FactsBook

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1998) Human Cell 11, 51-60. G. and Matsuo, Y. (1999) Leukemia 13, 835-842. G. C. (2000) In: The Encyclopedia of Cell Technology (Spier, E. ed). Wiley, New York, pp. 609-627. I. ed. (1993) Culture of Animal Cells. Wiley-Liss, New York. I. et al. eds. (1994) Culture of Hematopoietic Cells. Wiley-Liss, New York. B. G. (2000) In: Cell and Tissue Culture for Medical Research (Doyle, A. B. eds). John Wiley, New York (in press). E. ed. (1996) Human Cell Culture. Humana Press, Totowa, New Jersey. B. ed.

The top of the flask is slightly loosened to allow for free gaseous exchange into and out of the flask. The cells are fed by exchanging half of the culture volume with culture medium plus FBS at 2- to 3-day intervals. If the cells proliferate actively, the culture medium will soon change color due to a pH change caused by cellular metabolism. In this case, it is necessary to change the medium more frequently. Should the cells have doubled, the culture may be subdivided from the original flask into a second flask by diluting the suspension 1:2 with new medium.

1849-1880. J. et al. (1980) Proc. Natl. Acad. Sci. USA 77, 6815-6819. J. et al. (1980) Proc. Natl. Acad. Sci. USA 77, 7415-7419. Uchiyama, T. et al. (1977) Blood 50, 481-492. Hinuma, Y. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 6476-6480. Yoshida, M. et al. (1982) Proc. Natl. Acad. Sci. USA 79, 2031-2035. Chang, Y. et al. (1994) Science 266, 1865-1869. Schulz, T. et al. J. ed). ASM Press, Washington, pp. 87-134. Gaidano, G. et al. (1997) Leukemia Lymphoma 24, 257-266. G. et al. (1998) Leukemia 12, 1507-1517.

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